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Representative images of histological and immunohistochemical staining. (A) Masson's trichrome staining. (B) Haematoxylin and eosin staining. Immunostaining for p‐MLKL in patients with dilated cardiomyopathy (C–E) and a normal control (F). (G) A representative image of a tissue incubated with rabbit immunoglobulins as a substitute for a primary antibody. (H) Immunostaining for <t>caspase‐8.</t>
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Representative images of histological and immunohistochemical staining. (A) Masson's trichrome staining. (B) Haematoxylin and eosin staining. Immunostaining for p‐MLKL in patients with dilated cardiomyopathy (C–E) and a normal control (F). (G) A representative image of a tissue incubated with rabbit immunoglobulins as a substitute for a primary antibody. (H) Immunostaining for <t>caspase‐8.</t>
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Representative images of histological and immunohistochemical staining. (A) Masson's trichrome staining. (B) Haematoxylin and eosin staining. Immunostaining for p‐MLKL in patients with dilated cardiomyopathy (C–E) and a normal control (F). (G) A representative image of a tissue incubated with rabbit immunoglobulins as a substitute for a primary antibody. (H) Immunostaining for caspase‐8.

Journal: ESC Heart Failure

Article Title: Enhanced nuclear localization of phosphorylated MLKL predicts adverse events in patients with dilated cardiomyopathy

doi: 10.1002/ehf2.14059

Figure Lengend Snippet: Representative images of histological and immunohistochemical staining. (A) Masson's trichrome staining. (B) Haematoxylin and eosin staining. Immunostaining for p‐MLKL in patients with dilated cardiomyopathy (C–E) and a normal control (F). (G) A representative image of a tissue incubated with rabbit immunoglobulins as a substitute for a primary antibody. (H) Immunostaining for caspase‐8.

Article Snippet: To analyse necroptotic signalling in cardiomyocytes, we used polyclonal antibodies against caspase‐8 (1:4, PA121140, Invitrogen) and those against p‐MLKL‐Ser358 (1:50, ab208909, Abcam) as primary antibodies. ab208909 is an azide‐free version of ab187091, a well‐characterized antibody for detection of human p‐MLKL‐Ser358.

Techniques: Immunohistochemical staining, Staining, Immunostaining, Incubation